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Image Search Results
Journal: Frontiers in Aging Neuroscience
Article Title: Royal Jelly Alleviates Cognitive Deficits and β-Amyloid Accumulation in APP/PS1 Mouse Model Via Activation of the cAMP/PKA/CREB/BDNF Pathway and Inhibition of Neuronal Apoptosis
doi: 10.3389/fnagi.2018.00428
Figure Lengend Snippet: RJ treatment reduced the brain Aβ burden of APP/PS1 mice. (A,B) The levels of SDS-soluble and -insoluble (formic acid soluble) Aβ were measured using enzyme-linked immunosorbent assays (ELISAs). (C) The Aβ plaques in the cortex and hippocampus were estimated after immunohistochemical (IHC) staining with the Aβ antibody (B-4), the number of plaques per view ( D,E ; cortex, 20× magnification; hippocampus, 40× magnification) and proportions of the positive area (F,G) were calculated, three sections per animal. Scale bar = 200 μm. The arrows point out the representative senile plaques (SPs). Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, n.s. non-significant. (H–L) Immunoblot analysis of β-secretase (BACE1), insulin-degrading enzyme (IDE), neprilysin (NEP) and lipoprotein receptor-related protein-1 (LRP-1) in the brain homogenates. n = 6–10 mice per group. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, n.s. non-significant.
Article Snippet: Briefly, sections were blocked with 3% BSA for 30 min, then incubated with primary antibodies overnight at 4°C, including BACE1 (1:100, Santa Cruz Biotechnology, USA),
Techniques: Immunohistochemical staining, Immunohistochemistry, Western Blot